Product Class: Other

Endoproteinase LysC
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Item# Description List Price Web Price Qty
P8109S Endoproteinase LysC - 20 ug $212.00
$190.80
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
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Companion Products

Item# Description List Price Web Price Qty
P8100S Endoproteinase GluC - 50 ug $110.00
$99.00
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P8101S Trypsin-ultra Mass Spectrometry Grade - 100 ug $117.00
$105.30
ADD TO CART

    P8109S-cutsite

    Product Introduction

    Endoproteinase LysC is a serine Endoproteinase which cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.

    • Analyze complex proteomes
    • Compatible for simultaneous co-digestion with Trypsin-ultra Mass Spectrometry Grade (NEB# P8101)
    • Suitable for both in-gel and solution digests
    • Supplied lyophilized
    • Optimal activity and stability for up to 12 months

    Product Information

    Description

    Endoproteinase LysC is a serine endoproteinase, isolated from Lysobacter enzymogenes, that cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.

    A 16 hour native LysC digestion of Issatchenkia orientalis Cytochrome c using a 1:50 enzyme to substrate ratio, followed by LC-MS with a Thermo™ LTQ Orbitrap XL, identified 79 peptides with 85% sequence coverage.

    Product Source

    Isolated from Lysobacter enzymogenes

    Reconstitution

    Endoproteinase LysC should be reconstituted in 200 μl double-distilled water to make a 100 ng/μl solution in 10 mM Tris-HCl, pH 8.0. Rapid autolysis is a function of enzyme concentration.
    Product Categories:
    Proteases Products,
    Glycobiology Products,
    Proteome Analysis Products
    Applications:
    Glycomics and glycoproteomics,
    Protein Digestion,
    Glycobiology & Proteomics
    ,
    Proteomics

    Advantages and Features

    Application Features

    • Digestion of proteins for proteomic analysis by Mass Spectrometry
    • Protein and peptide identification

    Properties & Usage

      Usage Concentration

      100 - ng/μl

      Storage Temperature

      -20°C

      Molecular Weight

      Apparent: 30000 daltons

      Product Notes

      1. Storage Conditions: Supplied freeze-dried from a Tris-HCl buffer. The solution can be stored at 4°C for several days or in single-use aliquots at –20°C for several months. Use only freshly reconstituted protease for best results.
      2. LysC is active over a wide pH range, pH 5-12, depending on the application. Ammonium bicarbonate buffer may also be used in place of Tris-HCl.

      Protocols, Manuals & Usage

      Protocols

      1. In-gel Digestion Protocol for Endoproteinase LysC (P8109)
      2. Typical Reaction Conditions for Endoproteinase LysC

      Tools & Resources

      Selection Charts

      • Protease Selection Chart
      • Substrate-based Ligase Selection Chart

      Faqs & Troubleshooting

      FAQs

      1. What buffer conditions can Endoproteinase LysC be used in? Are there any special applications for which LysC is especially good?
      2. Is there a specific application for which LysC is an especially good choice?
      3. How much Endoproteinase LysC do I really need to digest my protein?
      4. Can Endoproteinase LysC used in a double digest with Trypsin-ultra?

      Troubleshooting

      Quality, Safety & Legal

      Quality Assurance Statement

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

      Specifications

      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

      Certificate Of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

      Safety DataSheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

      Legal and Disclaimers

      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.