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XRN-1

Catalog # Concentration Size List Price Quantity Your Price
M0338L 1000 units/ml 100 units $603.00
$542.70
M0338S 1000 units/ml 20 units $152.00
$136.80
Catalog # Size List Price Your Price
M0338L 100 units $603.00
$542.70
M0338S 20 units $152.00
$136.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

XRN-1 is highly processive 5´→3´ exoribonuclease, requiring 5´ monophosphate. It also acts on 5´ monophosphate ssDNA with greatly reduced efficiency.

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of DNases and RNases
  • Not sure which RNase to choose? Learn more about the different RNases offered by NEB.
XRN-1 is highly processive 5´→3´ exoribonuclease, requiring 5´ monophosphate. It also acts on 5´ monophosphate ssDNA with greatly reduced efficiency.
Product Source
Purified from E. coli carrying a plasmid overexpressing the yeast XRN-1 gene (1).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  XRN-1 M0338SVIAL -20 1 x 0.02 ml 1,000 units/ml
  NEBuffer™ 3 B7003SVIAL -20 1 x 1.25 ml 10 X
  XRN-1 M0338LVIAL -20 1 x 0.1 ml 1,000 units/ml
  NEBuffer™ 3 B7003SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that digests 1 µg of phosphorylated yeast RNA in 60 minutes at 37°C.

Reaction Conditions

1X NEBuffer™ 3
Incubate at 37°C

1X NEBuffer™ 3
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

20 mM Tris-HCl
500 mM NaCl
2 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% (w/v) Triton® X-100



Companion Products
Materials Sold Separately
Notes
  • We recommend adding Murine RNase Inhibitor (NEB #M0314) in your reaction at 1 unit/µl to prevent non-specific RNA degradation.
References
  • Stevens, A (1980). Biol. Chem. 255, 3080-3085.
FAQs
Tech Tips
  • We strongly recommend wearing gloves, using nuclease-free tubes and reagents, and thoroughly cleaning pipettes and bench surfaces to avoid RNase contamination.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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