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Sce PUS1

Catalog # Concentration Size List Price Quantity Your Price
M0526S 100 pmol/μl 5000 pmol $123.00
$110.70
Catalog # Size List Price Your Price
M0526S 5000 pmol $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Sce PUS1 isomerizes uridine to pseudouridine in predominantly single-stranded RNA in vitro
  • Sce PUS1 is a stand-alone enzyme and does not require an RNA guide or co-factor to modify RNA
  • Sequence-specific pseudouridine modification by Sce PUS1 is an alternative to randomly incorporated modified nucleosides by RNA polymerases

This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.

 
Sce Pseudouridine Synthase I (Sce PUS1) converts Uridine to Pseudouridine in single-stranded RNA, with a preference for Uridines in single-stranded RNA regions over Uridines in double-stranded RNA. The optimal substrate is an unstructured RNA that is 15 nt long or longer.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Sce PUS1 M0526SVIAL -20 1 x 0.05 ml 100 pmol/μl
  NEBuffer™ r1.1 B6001SVIAL -20 1 x 1.25 ml 10 X
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
References
  • Arluison, V., Buckle, M., & Grosjean, H. (1999). Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA.  J Mol Bio. 289 (3), 491-502.
  • Arluison, V., Batelier, G., Riès-Kautt, M., & Grosjean, H. (1999). RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA (Phe). Biochimie. 81 (7), 751-756.
  • Carlile, T. M., Martinez, N. M., Schaening, C., Su, A., Bell, T. A., Zinshteyn, B., & Gilbert, W. V (2019). mRNA structure determines modification by pseudouridine synthase 1. Nature Chemical Biolog. 15 (10), 966-974.
  • (2014). Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells. Nature. 515 (725), 143-146.
  • (1998). The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.. 4 (7), 856-869.
  • Grünberg S, Doyle LA, Wolf EJ, Dai N, Corrêa IR Jr, Yigit E, et al (2023). The structural basis of mRNA recognition and binding by yeast pseudouridine synthase PUS1. 18(11), DOI: e0291267, PLoS ONE.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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