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Nuclease P1

Catalog # Concentration Size List Price Quantity Your Price
M0660S 100000 units/ml 10000 units $83.00
$74.70
Catalog # Size List Price Your Price
M0660S 10000 units $83.00
$74.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • Single-stranded (ssDNA or RNA) specific endonuclease
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Nuclease P1 is ideal for:

  • Conversion of single-stranded DNA or RNA to 5'-mononucleotides
  • Analysis of the base composition of nucleic acids
  • Studies of the potential damage and modification in DNA
Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    What are exonucleases and their applications?
Nuclease P1 is a zinc-dependent single-strand specific nuclease which hydrolyzes phosphodiester bonds in RNA and ssDNA with no base specificity. Nuclease P1 also exhibits 3′-phosphomonoesterase activity (1). Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer 1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.
Product Source
Isolated from Penicillium citrinum.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Nuclease P1 M0660SVIAL -20 1 x 0.1 ml 100,000 units/ml
  Nuclease P1 Reaction Buffer B0660SVIAL -20 2 x 1 ml 10 X
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Notes
  • Substrate specificity for Nuclease P1 is as follows: 3´ AMP > RNA > ssDNA >> dsDNA.
  • The rate of hydrolysis of 2´-AMP is 3,000-fold less than that of 3´-AMP.
  • Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer 1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.
References
  • Fujimoto, M., Kuninaka, A. and Yoshino, H. (1974). Agric. Biol. Chem. 38, 777-783.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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