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T5 Exonuclease

Catalog # Concentration Size List Price Quantity Your Price
M0663L 10000 units/ml 5000 units $438.00
$394.20
M0663S 10000 units/ml 1000 units $109.00
$98.10
Catalog # Size List Price Your Price
M0663L 5000 units $438.00
$394.20
M0663S 1000 units $109.00
$98.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

  • Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease
  • Initiates at the 5' termini of linear or nicked double-stranded DNA
  • Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

T5 Exonuclease is ideal for:

  • Removal of incomplete ligation products from ligated circular double-stranded DNA
  • Degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning
  • Degradation of contaminating linear and nicked DNA in plasmid samples
Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    What are exonucleases and their applications?
  • Monarch_MiniprepProtocol_VideoThumb
    NEB®: Exonucleases and Endonucleases as Molecular Tools Webinar
T5 Exonuclease degrades DNA in the 5´ to 3´ direction (1). T5 Exonuclease is able to initiate nucleotide removal from the 5′ termini or at gaps and nicks of linear or circular dsDNA (1). However, the enzyme does not degrade supercoiled dsDNA (2). T5 Exonuclease also has ssDNA endonuclease activity.
Product Source
The T5 phage D15 gene is expressed as His-tag fusion in E. coli.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  T5 Exonuclease M0663SVIAL -20 1 x 0.1 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
  T5 Exonuclease M0663LVIAL -20 1 x 0.5 ml 10,000 units/ml
  NEBuffer™ 4 B7004SVIAL -20 1 x 1.25 ml 10 X
Application Features
  • Degradation of linear ssDNA, dsDNA or nicked plasmid DNA while maintaining supercoiled plasmid DNA.
  • Remove denatured DNA isolated during alkaline lysis-based plasmid purification procedure (5).
  • Enhance the transfection efficiency of miniprep from plasmid cDNA libraries (6).
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
Materials Sold Separately
References
  • Sayers, J.R. et al. (1991). Nucleic Acids Res. 19, 4127-4132.
  • Sayers, J.R. et al. (1990). J. of Biol. Chem. 265, 18311-18317.
  • Kaliman, A.V. et al. (1986). FEBS. 195, 61-64.
  • Frenkel, G.D. et al. (1971). J. of Biol. Chem. 246, 4839-4847.
  • Sayers, J.R. et al. (1996). Analytical Biochem. 241, 186-189.
  • Kiss-Toth, E. et al. (2001). Analytical Biochem. 288, 230-232.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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