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dUTP Solution

Catalog # Concentration Size List Price Quantity Your Price
N0459S 100 mM 25 µmol $102.00
$91.80
Catalog # Size List Price Your Price
N0459S 25 µmol $102.00
$91.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • ≥ 99% purity as determined by HPLC analysis
  • For use with polymerases such as Taq DNA Polymerase (NEB# M0273) or Q5U® Hot Start High-Fidelity DNA Polymerase (NEB# M0515)
  • Combine with Antarctic Thermolabile UDG (NEB# M0372) to prevent carryover contamination in PCR and qPCR-related technologies
 

Contains 0.25 ml of 100 mM ultrapure dUTP. dUTP can either be fully substituted for dTTP or added in addition to conventional dNTPs. dUTP can be used with Family A polymerases (such as Taq) or modified Family B polymerases, such as Q5U Hot Start High-Fidelity DNA Polymerase. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

For a simplified protocol, dUTP can be added to most polymerase reactions at the same concentration as other nucleotides (e.g., a 1:1:1:1:1 dA/dC/dG/dT/dU ratio). 

For applications that require other molar ratios (up to and including full substitution of dU for dT), custom nucleotide mixes must be made. We recommend starting with a concentration of 0.2 mM of each dNTP for both Taq and Q5U DNA polymerases.

Supplied in: 
Ultra purified water as a sodium salt at pH 7.5.

Concentration: 
The dUTP Solution is supplied at a concentration of 100 mM (+/- 5 mM) as determined by A260 absorbance.

Diluent Compatibility: 
Can be diluted using sterile distilled water, preferably Milli-Q® water or can be diluted using sterile TE [10 mM Tris-HCl, 1 mM EDTA (pH 7.5)].

 
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  dUTP Solution N0459SVIAL -20 1 x 0.25 ml 100 mM
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us atgbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications
 

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