Expires Dec 31st, 2023
A Rapid 1-Day Cloning to
Protein Expression Workflow

Molecular biology tools enable the custom generation of proteins with complete control of sequence, purification tags, secretion signals, and other performance characteristics. While the breadth of tools allows researchers to create their desired protein, this process often involves a low-throughput and time-consuming, multiday workflow using live cells. To overcome these limitations, we have demonstrated a completely in vitro workflow that

combines Golden Gate DNA Assembly, rolling circle amplification (RCA), and cell-free protein expression (CFPE) to rapidly screen the impact of multiple protein designs simultaneously. This workflow enables researchers to generate an array of protein variants in as little as a single day using a basic set of custom DNA vectors or insertion fragments. It also provides a means to assess the engineering constraints that are unique to each protein of interest and allows for the rapid identification of soluble protein.

Product Listing

Search catalog# or product name to sort:

Catalog # Size Product Name Quantity Your Price  
E1603L 500 reactions phi29-XT RCA Kit
E1603S 100 reactions phi29-XT RCA Kit
E5360L 100 reactions NEBExpress™ Cell-free E. coli Protein Synthesis System
E5360S 10 reactions NEBExpress™ Cell-free E. coli Protein Synthesis System
M1100L 250 reactions NEBridge™ Ligase Master Mix
M1100S 50 reactions NEBridge™ Ligase Master Mix
R3733L 5000 units BsaI-HF®v2
R3733S 1000 units BsaI-HF®v2
S1423L 5 ml Ni-NTA Magnetic Beads
S1423S 1 ml Ni-NTA Magnetic Beads
S9145S 2 ml SNAP-Capture Magnetic Beads
T1020L 250 preps Monarch® DNA Gel Extraction Kit
T1020S 50 preps Monarch® DNA Gel Extraction Kit
*TERMS & CONDITIONS: Offer valid in Canada only. Expires Dec 31th, 2023. Discount is eligible for products listed on this flyer. Purchase can be made online at www.neb.ca or through a NEB freezer program. Eligible products get discounted automatically when added to cart. Promotion not valid for cash or cash equivalent towards purchase(s). No substitutions. Offer may not be applied to existing, pending or prior orders. Cannot be combined with any other promotion or discount. One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at orders.ca@neb.com