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FAQ: What is the activity of Thermostable FEN1 at various temperatures?

 TEMPERATURE  % ACTIVITY
 25°C < 1%
 37°C < 5%
 45°C  20%
 50°C  50%
 60°C  90%
 65°C  100%
 70°C  95%

*Incubation time can be increased to obtain enhanced FEN1 cleavage at non-ideal reaction temperatures
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