No. Standard, desalted primers may be used.
For more information, please download the application note, Construction of an sgRNA-Cas9 expression vector via single-stranded DNA oligo bridging of double-stranded DNA fragments.
No. Standard, desalted primers may be used.
For more information, please download the application note, Construction of an sgRNA-Cas9 expression vector via single-stranded DNA oligo bridging of double-stranded DNA fragments.