FAQ: Why is induced protein insoluble (C2523)?

Check for insolubility - this is important because high expression and high production of protein can result in the target protein becoming insoluble. Potential solutions for this are:
» Induce at lower temperatures (as low as 15°C overnight)
» Reduce IPTG concentration to between 0.01 mM and 0.1 mM
» Induce for less time (as little as 15 minutes)
» Induce earlier in growth (OD600 = 0.3 or 0.4)
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