SplintR Ligase is extremely effective at sealing nicks between adjacent
residues of DNA strands hybridized to RNA splints or bridges, with an efficiency often 10-100 times that seen for T4 DNA ligase. On a fully-DNA
substrate, this nick-sealing activity is equivalent to other DNA ligases. It is not a good choice for molecular cloning workflows in which double-strand breaks need to be repaired (such as joining a vector and an insert).