Yes. However, the optimal concentration of each oligonucleotide should be determined. As a starting point, we recommend using 45 nM of each oligonucleotide that is less than or equal to twelve 60-base oligonucleotides containing 30-base overlaps.
For more information, please download the application note, Construction of an sgRNA-Cas9 expression vector via single-stranded DNA oligo bridging of double-stranded DNA fragments.