FAQ: What is the optimal heat shock time for the 384-well plate format NEB 5-alpha Competent E. coli (NEB #C2987R)?
Both the temperature and the timing of the heat shock step are important and specific to the transformation volume and vessel. Using the 384-well plate provided, 15 seconds at 42°C is optimal. Expect approximately 40% loss in transformation efficiency when heat shocking for 20 seconds.