FAQ: Can HiFi Taq DNA Ligase buffer be used with other DNA ligases?
Yes, HiFi Taq DNA Ligase buffer can be used with Taq DNA Ligase. Use of the HiFi Taq DNA Ligase buffer with Taq DNA Ligase will result in a modest fidelity and activity increase. ATP-dependent ligases (for example, 9°N™ DNA Ligase NEB #M0238 , T4 DNA Ligase NEB #M0202 and T7 DNA Ligase NEB #M0318 ) are incompatible with this buffer.