The optimal pH for the cleavage of fucosylated-biantennary (i.e. porcine fibrinogen) and triantennary glycans (i.e. fetuin) by Endo F3 is pH 4.5 (10X Glycobuffer 4). However, fucosylated-biantennary glycans can also be cleaved by Endo F3 at pH 6.0 (10X Glycobuffer 3).