FAQ: Can SplintR® DNA Ligase be used for ligation-based methods of microRNA detection?
Yes, SplintR DNA Ligase has been show to be suitable for detection of microRNA by qPCR-coupled ligase detection reaction, and for detection using circularizable Padlock probes. SplintR Ligase overcomes the efficiency limitation of T4 DNA ligase for in vitro or direct in situ RNA detection by ligation, making SplintR Ligase an attractive tool for the detection and quantification of microRNA.