FAQ: How much Endo F2 should I use to deglycosylate a glycoprotein under native conditions?
We recommend using 1µl of Endo F2 per 20µg of native glycoprotein. Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
Combine 20 µg of glycoprotein, 1 µL of 10X Glycobuffer 4 and H20 (if necessary) to make a 10 µL total reaction volume.