FAQ: What additional substrates are hydrolyzed by EGCase I?

See substrates listed in Table 1 below, which was adapted from: Albrecht, S. et al. (2016) Analytical Chemistry 88, 4795-4802.

Table 1. Substrate specificity of recombinant Endoglycoceramidase I
The table below lists the quantities of glycans released from glycolipid standards (2 nmol) using 2 mU R. triatomea rEGCase I following incubation at 37°C for 16 hours.

Substrate
Glycolipid Structure
Hydrolysis
%

Gangliosides    
 GM3  Neu5Acα2-3Galβ1-4Glcβ1-1'Cer 100
 GM1a  Galβ1-3GalNAcβ1-4(Neu5Acα2-3)
Galβ1-4Glcβ1-1'Cer
 100
 FGM1 Fucα1-2Galβ1-3GalNAcβ1-4
(Neu5Acα2-3)Galβ1-4Glcβ1-1'Cer
 100
 GD3 Neu5Acα2-8Neu5Acα2-3Galβ1-4
Glcβ1-1'Cer
100
 GD1a  Neu5Acα2-3Galβ1-3GalNAcβ1-4
(Neu5Acα2-3)Galβ1-4Glcβ1-1'Cer
100
 GD1b Galβ1-3GalNAcβ1-4(Neu5Acα2-8
Neu5Acα2-3)Galβ1-4Glcβ1-1'Cer
100
 GT1b Neu5Acα2-3Galβ1-3GalNAcβ1-4
(Neu5Acα2-8Neu5Acα2-3)Galβ1-4Glcβ1-1'Cer
 100
     
 Globosides    
Gb4
GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1'Cer
 100
     
 Cerebrosides    
 GlcCer Glcβ1-1'Cer
100
 GalCer Galβ1-1'Cer
 5-10
 Sulfatide  HSO3-3Galβ1-1'Cer  0
 Psychosine  Galβ1-1'sphingosine  0
 LacCer  Galβ1-4Glcβ1-1'Cer  100
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