FAQ: What applications are Thermostable RNase H suitable for?

Thermostable RNase H can be used as a replacement for E. coli RNase H in assays such as RNA structure mapping, site-specific cleavage of RNA, isothermal amplification methods, removal of mRNA during second strand cDNA synthesis, and removal of poly(A) tails on mRNAs when hybridized to oligo(dT).  Due to the increased stability of Thermostable RNase H at higher temperatures, assays requiring higher stringency may benefit from the use of this enzyme.

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