FAQ: Does Hi-T7 RNA Polymerase recognize the same promoter sequence as the wild-type T7 RNA Polymerase from bacteriophage?
Yes. Hi-T7 and T7 RNA Polymerases recognize the same promoter:
5′ TAATACGACTCACTATAG 3′ with transcription starting at the G.
The first base in the transcript will be a G.