Elution of large amounts of RNA (> 50 µg) can be maximized by incubating the elution buffer (nuclease-free water) with the column for 5 minutes at room temperature prior to spinning. Decreasing the incubation time will decrease RNA recovery.
Elution of large amounts of RNA (> 50 µg) can be maximized by incubating the elution buffer (nuclease-free water) with the column for 5 minutes at room temperature prior to spinning. Decreasing the incubation time will decrease RNA recovery.