FAQ: Is antibiotic selection necessary to maintain the MiniF-lacIq plasmid?

Although the MiniF plasmid carries the chloramphenicol resistance gene, addition of chloramphenicol is not necessary.  If added, chloramphenicol should be at a final concentration of 10 μg/ml.  Chloramphenicol levels greater than 15 μg/ml will impair growth.
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