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FAQ: Is Tte UvrD Helicase compatible with the WarmStart Colorimetric LAMP 2X Master Mix (NEB #M1800)?

No, Tte UvrD Helicase should not be used with Colorimetric LAMP. As the UvrD acts on DNA substrates it hydrolyzes dATP, releasing an H+ with each hydrolysis much like a DNA polymerase incorporating dNTP. This proton production will result in a drop in pH for the colorimetric LAMP solution independent of any amplification reaction, changing the color of all reactions containing the WarmStart Colorimetric LAMP 2X Master Mix and oligonucleotide primers.
 
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