FAQ: What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?

POLYMERASE PRODUCTS

FIDELITY*

ERROR RATE

PRODUCT ENDS

MAX PRODUCT LENGTH**

EXTENSION TEMPERATURE

MODIFIED NUCLEOTIDE INCORPORATION***

URACIL INCORPORATION

5´-3´ EXONUCLEASE

3´-5´ (PROOFREADING) EXONUCLEASE

Q5 Polymerases

280X

<0.44 x 10-6

Blunt

20kb simple, 10kb complex

72°C

5mC, 5hmC, 6mA

No (except Q5U)

-

++++

Phusion Polymerases

39-50X

0.44 x 10-6

Blunt

20kb simple, 10kb complex

72°C

5mC, 5hmC

No

-

++++

OneTaq Polymerases

2X

<140 x10-6

3´A/blunt

6kb

68°C

5mC, 5hmC, biotin, DIG

Yes

+

++

Taq Polymerases

1X

2.85 x 10^-4

3´A

5kb

68°C

5mC, 5hmC, biotin, DIG

Yes

+

-

Hemo KlenTaq
 nt  nt 3´A 2kb  68°C
  Yes No -
LongAmp® Polymerases
2X
  3´A/blunt
30kb
65°C
  No
Yes
++

*Fidelity relative to Taq DNA polymerase. We continue to investigate assays to characterize Q5's very low error rate to ensure that we present the most accurate fidelity data possible (Potapov, V, and Ong, J.L. (2017) PloS ONE, 12(1): e0169774).   
                                    
**Simple templates include plasmid, viral and E. coli genomic DNA. Complex templates include plant, human and other mammalian genomic DNA and cDNA.

*** For more information, contact Technical Support at info@neb.com   

For more information on properties to help you select a polymerase for your application, please see our DNA Polymerase Selection Chart.


Learn More
 
Fidelity and error rate

The fidelity of a DNA polymerase is defined by its ability to accurately replicate a template, while error rate is the rate of misincorporation of an incorrectly matched nucleotide. Fidelity is important for applications in which the DNA sequence must be correct after amplification.
 
To learn more about how fidelity is measured, click here.
 
Product ends and exonuclease activity

Check out the "Learn More" section on our PCR Product Ends FAQ and our Exonuclease Activity for DNA Polymerases FAQ.


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