No, E5hmC-seq libraries specifically detect 5hmC. Furthermore, unmethylated cytosines and 5mC cannot be distinguished. To identify only 5mC, users need to prepare two libraries and then perform a subtractive data analysis. EM-seq™ libraries detect 5mC and 5hmC whereas E5hmC-seq libraries identify 5hmC. Subtracting E5hmC-seq data from EM-seq data therefore provides 5mC information.