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Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Catalog # Concentration Size List Price Quantity Your Price
M0320L 5000 units/ml 2000 units $395.00
$355.50
$237.00
M0320S 5000 units/ml 400 units $99.00
$89.10
$59.40
Catalog # Size List Price Your Price
M0320L 2000 units $395.00
$355.50
$237.00
M0320S 400 units $99.00
$89.10
$59.40
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The industry standard

  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • Mg-free buffer offers flexibility to adjust magnesium concentrations for use with microarray or DHPLC applications
  
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 

It is supplied with 10X Standard Taq (Mg-free) Reaction Buffer and MgCl2. 10X Standard Taq (Mg-free) Reaction Buffer is detergent-free and designed to be compatible with existing assay systems.
Highlights
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Robust and reliable reactions
  • Tolerates a wide range of templates
  • Incorporates dUTP, dITP and fluorescently-labeled nucleotides
Product Source
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Taq DNA Polymerase with Standard Taq (Mg-free) Buffer M0320SVIAL -20 1 x 0.08 ml 5,000 units/ml
  Standard Taq (Mg-free) Reaction Buffer Pack B9015SVIAL -20 2 x 1.5 ml 10 X
  Magnesium Chloride (MgCl2) Solution B9021SVIAL -20 1 x 1.5 ml 25 mM
  Taq DNA Polymerase with Standard Taq (Mg-free) Buffer M0320LVIAL -20 1 x 0.4 ml 5,000 units/ml
  Standard Taq (Mg-free) Reaction Buffer Pack B9015SVIAL -20 4 x 1.5 ml 10 X
  Magnesium Chloride (MgCl2) Solution B9021SVIAL -20 5 x 1.5 ml 25 mM
Application Features
  • PCR
  • Primer Extension
  • DHPLC
  • Microarray Analysis
  • Colony PCR

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X Standard Taq (Mg-free) Reaction Buffer Pack
Supplement with 1.5 mM MgCl2 solution

1X Standard Taq (Mg-free) Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
(pH 8.3 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween® 20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 94000 daltons

5' - 3' Exonuclease

Yes

3' - 5' Exonuclease

No

Strand Displacement

+

Resulting Ends

  • Single-base 3´ Overhangs

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. 

Error Rate

~ 285x10-6bases

Companion Products
Materials Sold Separately
Notes
  • 5'→3' flap endonuclease destroys displaced strand.
References
  • Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
  • Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
  • Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
  • Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). NucleicAcids Res.. 18, 7317-7322.
  • Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
  • Saiki R.K. et al. (1985). Science. 230, 1350-1354.
  • Powell, L.M. et al. (1987). Cell. 50, 831-840.
  • Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  • Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). NucleicAcids Res.. 18, 7465.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.NEW ENGLAND BIOLABS®, QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas, LLC.

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