Endoglycoceramidase I (EGCase I)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
P0773S	6 units/ml	150 milliunits	$120.00		$108.00

Catalog #	Size	List Price	Your Price
P0773S	150 milliunits	$120.00	$108.00

Endoglycoceramidase I (EGCase I) catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids. The enzyme leaves the oligosaccharide and ceramide intact, keeping them suitable for further analysis. The enzyme efficiently releases oligosaccharides from ganglio- and globo-type glycosphingolipids, certain type of cerebrosides (glucosylcerebrosides), and exhibits low activity towards galactocerebrosides (See Reference 1, FAQ #3). EGCase I does not hydrolyze sulfatides nor psychosine.

Product Source

EGCase I is isolated from a strain of E. coli, which contains the cloned EGCase I gene from Rhodococcus triatomea.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

P0773S -20

	Endoglycoceramidase I (EGCase I)	P0773SVIAL	-20	1 x 0.025 ml	6 units/ml
	EGCase I Reaction Buffer	B0773SVIAL	-20	1 x 1.5 ml	10 X

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

References

Albrecht, S. et al. (2016). Analytical Chemistry. 88, 4795-4802.

Protocols

Protocol for use with EGCase I (P0773)

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

P0773S_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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