Don’t be biased! The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina offers an amplification-free workflow for DNA-seq based on the streamlined and reliable Ultra II FS workflow. Starting with as little as 50 ng of DNA, get the high-quality, high-yield libraries that you need without PCR bias.
For convenience, a version of this kit is available with SPRIselect beads.
Note: For sheared DNA, we recommend the NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina.
The NEBNext Ultra II FS DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. The fast, user-friendly workflow includes reliable enzymatic DNA fragmentation, requires minimal hands-on time, resulting in excellent library yields and GC coverage without requiring PCR.
Advantages
A separate version of the NEBNext Ultra II FS DNA PCR-free Library Prep Kit is available with sample purification beads.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E7430S | -20 |
NEBNext® Ultra™ II FS Enzyme Mix | E7806AVIAL | -20 | 1 x 0.048 ml | ||
NEBNext® Ultra™ II FS Reaction Buffer | E7807AVIAL | -20 | 1 x 0.168 ml | ||
NEBNext® Ligation Enhancer | E7374AVIAL | -20 | 1 x 0.024 ml | ||
NEBNext® Ultra™ II Ligation Master Mix | E7648AVIAL | -20 | 1 x 0.72 ml | ||
TE Buffer (1X) | E7808AVIAL | -20 | 1 x 1.1 ml | 1 X | |
DTT | E7139AAVIAL | -20 | 1 x 0.5 ml | 100 mM |
E7430L | -20 |
NEBNext® Ultra™ II FS Enzyme Mix | E7806AAVIAL | -20 | 1 x 0.192 ml | ||
NEBNext® Ultra™ II FS Reaction Buffer | E7807AAVIAL | -20 | 1 x 0.672 ml | ||
NEBNext® Ligation Enhancer | E7374AAVIAL | -20 | 1 x 0.096 ml | ||
NEBNext® Ultra™ II Ligation Master Mix | E7648AAVIAL | -20 | 3 x 0.96 ml | ||
TE Buffer (1X) | E7808AAVIAL | -20 | 1 x 4.3 ml | 1 X | |
DTT | E7139AAVIAL | -20 | 1 x 0.5 ml | 100 mM |
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