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RNA 5' Pyrophosphohydrolase (RppH)

Catalog # Concentration Size List Price Quantity Your Price
M0356S 5000 units/ml 200 units $157.00
$141.30
Catalog # Size List Price Your Price
M0356S 200 units $157.00
$141.30
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

RNA 5´ Pyrophosphohydrolase (RppH) removes pyrophosphate from the 5´ end of triphosphorylated RNA to leave a 5´ monophosphate RNA.

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of endonucleases, exonucleases, RNases and phosphatases
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  • Monarch_MiniprepProtocol_VideoThumb
    Avoiding RNase Contamination
The bacterial RNA 5´ Pyrophosphohydrolase (RppH) removes pyrophosphate from the 5´ end of triphosphorylated RNA to leave a 5´ monophosphate RNA (1). The RppH protein was also known as NudH/YgdP which can split Ap5A to ADP and ATP (2).
Product Source
An E. coli strain containing a clone of the E. coli RppH gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  RNA 5' Pyrophosphohydrolase (RppH) M0356SVIAL -20 1 x 0.04 ml 5,000 units/ml
  NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage

Unit Definition

One unit is the amount of enzyme that converts 1 µg 300 mer RNA transcript into a XRN-1 digestible RNA in 30 minutes at 37°C.

Reaction Conditions

1X NEBuffer™ 2
Incubate at 37°C

1X NEBuffer™ 2
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

20 mM Tris-HCl
200 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.01% Triton® X-100
pH 7.5 @ 25°C



Materials Sold Separately
Notes
  • Since RppH is active in the presence of Mg2+, it can be incubated with RNA Ligase or XRN-1.
References
  • Deana, A. et al. (2008). Nature. 451, 355-358.
  • Bessman, M.J. et al. (2001). JBC. 276, 37834.
  • Neri F, Rapelli S, Krepelova A, Incarnato D, Parlato C, Basile G, et al. (2017). Intragenic DNA Methylation prevents spurious transcription initiation. Nature. 543, 72–7.
  • Hetzel, Jonathan et al. (2016). Nascent RNA Sequencing Reveals Distinct Features in Plant Transcription. Proceeding of the National Academy of Sciences. 113.43, 12316–12321.
FAQs
Tech Tips
  • We strongly recommend wearing gloves, using nuclease-free tubes and reagents, and thoroughly cleaning pipettes and bench surfaces to avoid RNase contamination.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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