HeLa Genomic DNA

Catalog # Concentration Size List Price Quantity Your Price
N4006S 100 µg/ml 15 µg
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Catalog # Size List Price Your Price
N4006S 15 µg
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Highly pure HeLa Genomic DNA for use in a wide variety of applications including: PCR, SNP analysis and southern blotting
  • Genomic DNA library construction
  • Control DNA for Methylation-specific PCR (MSP), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (MS-SNuPE,) Combined Bisulfite Restriction Analysis (COBRA), Bisulfite treatment and PCR-single strand Confirmation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS)
Human female HeLa genomic DNA.

Product Source:
 
HeLa (cervix adenocarcinoma) cells were grown to confluency in DMEM plus 10% fetal bovine serum. Genomic DNA was isolated by a standard genomic purification protocol (1), phenol extracted and equilibrated to 10 mM TrisHCl (pH 7.5) and 1 mM EDTA.
Application Features
  • A control for Methylation-Specific PCR (MSP) (2), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE), Combined Bisulfite Restriciton Analysis (COBRA), Bisulfite treatment and PCR-Single-Strand Conformation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS).
  • PCR, SNP Analysis, Southern Blotting
  • Genomic DNA library construction

Properties & Usage

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C



Companion Products
Notes
  • Avoid multiple freeze/thaw cycles.
References
  • Sambrook, J. and Russell, D. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.), (pp. 6.4-6.12). Cold Spring Harbor: Cold Spring Ha.
Quality Control Assay
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Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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