FAQ: What is the minimum elution volume that can be used with the Monarch® Genomic DNA Purification Kit columns?
The minimum elution volume is 35 µl. For maximum recovery, 100 µl is recommended. Smaller elution volumes will lead to more concentrated samples but also a lower yield. Typically, when 35 µl is used for elution, yields are approximately 20% lower than when using 100 µl. Using elution volumes lower than 35 µl will result in inconsistent wetting of the silica matrix and significantly reduced recovery.
For more information, see “Considerations for Elution and Storage” in the product manual.