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Alkaline Phosphatase, Calf Intestinal (CIP)

Catalog # Concentration Size List Price Quantity Your Price
M0290L 10000 units/ml 5000 units
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M0290S 10000 units/ml 1000 units
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Catalog # Size List Price Your Price
M0290L 5000 units
M0290S 1000 units
Please Inquire
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Alkaline Phosphatase, Calf Intestinal (CIP) nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters.

  • Faster reaction setup (no supplemental additives like zinc required)
  • Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
  • Less enzyme required (high specific activity), resulting in a lower cost per reaction
  • No need for multiple phosphatases (CIP removes 5´- and 3´- phosphates from DNA, RNA and dNTPs)
  • Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis

Storage Temperature:  -20°C

 
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    The Mechanism of DNA Phosphorylation
Alkaline Phosphatase, Calf Intestinal (CIP) nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. Also, CIP hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). CIP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5´ protruding, 5´ recessed and blunt ends. CIP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.
Product Source
Calf intestinal mucosa
Features
  • Faster reaction setup (no supplemental additives like zinc required)
  • Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
  • Less enzyme required (high specific activity), resulting in a lower cost per reaction
  • No need for multiple phosphatases (CIP removes 5´- and 3´- phosphates from DNA, RNA and dNTPs)
  • Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
Application Features
  • Dephosphorylation 5´ and 3´ ends of DNA and RNA.
  • Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.
  • Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase.
  • Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis.
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products
Notes
  • CIP, as are most alkaline phosphatases, isa Zn2+ and Mg2+-dependent enzyme. Ourformulation of its storage buffer provides Zn2+and Mg2+, which does not require supplementalzinc or other additives in reactions with CIP.

  • CIP is also active in 1X NEBuffers 1.1, 2.1,3.1, as well as NEBuffers 1, 2, 3, 4 and uniqueNEBuffer for EcoRI.

  • CIP activity is enhanced in the presence ofmonovalent salts.

  • CIP is inhibited by metal chelators (e.g. EDTA),inorganic phosphate and phosphate analogs.
  • CIP activity is decreased in the presence of reducing agents (DTT, β-ME).
References
  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.). (p 5.72), Cold Spring Harbor Laboratory Press.
  • Mossner, E., Boll, M. and Pfleiderer, G. (1980). Hoppe Seylers Z. Physiol Chem. 361,
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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