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Thermostable OGG

Catalog # Concentration Size List Price Quantity Your Price
M0464S 8000 units/ml 500 units $113.00
$101.70
Catalog # Size List Price Your Price
M0464S 500 units $113.00
$101.70
Catalog #
Qty:
 
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M0464
  • Thermostable oxoguanine glycosylase
  • Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
  • N-glycosylase activity releases 8-oxo-7,8-dihydroguanine (8-oxoG), generating an AP site. The AP-lyase activity cleaves 3′ to the AP site leaving a 5′ phosphate and a 3′-phospho-α, β-unsaturated aldehyde.
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 
 
Thermostable OGG is an archaeal 8-oxoguanine (8-oxoG) DNA glycosylase which acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases the damaged purine (8-oxoguanine) from double stranded DNA, generating an apurinic (AP) site. The AP-lyase activity cleaves 3′ to the AP site leaving a 5′ phosphate and a 3′-phospho-α, β-unsaturated aldehyde. Unlike some other DNA glycosylases, Thermostable OGG specifically recognizes and cleaves only 8-oxoG and no other modified bases.  

Figure 1: Unlike Fpg, Thermostable OGG specifically recognizes and cleaves only 8-oxoguanine and no other modified bases. 



The ability to excise modified bases was queried by incubating Thermostable OGG (8 U) or Fpg (8 U) with fluorescently labeled dsDNA containing modified bases (either 8-oxoadenine, 5-hydroxycytosine, thymine glycol, 5-hydroxyuridine or 8-oxoguanine). Samples were analyzed by capillary electrophoresis and cleavage products are evidenced by the appearance of a cleaved product peak. Thermostable OGG only cleaves the 8-oxoguanine sample (top panel) while Fpg is active on other substrates including , 5-hydroxycytosine, thymine glycol and  5-hydroxyuridine (bottom panel).

Highlights
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
Product Source
An E. coli strain that carries a cloned thermostable archael oxoguanine glycosylase gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Thermostable OGG M0464SVIAL -20 1 x 0.065 ml 8,000 units/ml
  rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Materials Sold Separately
Quality Control Assay
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Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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