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N-Glycopeptide Binding Protein

Catalog # Concentration Size List Price Quantity Your Price
P0872S 1 mg $477.00
$429.30
Catalog # Size List Price Your Price
P0872S 1 mg $477.00
$429.30
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Enrich for N-glycopeptides in complex proteomics samples with this engineered and improved lectin without requiring the use of HILIC columns or cartridges.
  • Significantly improved N-glycopeptide spectra match and glycoprotein identification and discovery.
  • Broad specificity to high mannose, complex, and hybrid N-glycans, as well as fucosylated and non-fucosylated, sialylated and non-sialylated, with bi-, tri- and tetra-antennary structures.
  • Recombinant and free of protease and glycosidase activities.
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N-Glycopeptide Binding Protein, also known as Fbs1-GYR lectin, is engineered to selectively bind and enrich a wide variety of N-glycopeptides in a complex proteomic sample to assist N-glycoprotein identification and discovery, without requiring the use of traditional lectin affinity chromatography (LAC) techniques, HILIC columns or cartridges. This engineered lectin, originating from human Fbs1 protein, exhibits improved specificity towards high mannose, complex, and hybrid N-glycans, as well as towards fucosylated and non-fucosylated, sialylated and non-sialylated, with bi-, tri- and tetra-antennary structures. N-Glycopeptide Binding Protein includes a SNAP-tag for immobilizing to create versatile workflows.
Product Source
An E. coli strain that carries human Fbs1 mutant gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  N-Glycopeptide Binding Protein P0872SVIAL -20 1 x 1 ml 1 mg/ml
Features
  • Engineered lectin, a glycan-binding protein that recognizes a wide variety of N-glycoforms, for enrichment and identification of glycopeptides and glycoproteins
  • Selectively binds and enriches N-glycopeptides in a complex proteomics sample, unlike other lectin affinity chromatography (LAC) techniques, HILIC, or other lectin-based enrichment methods
  • Significantly increased glycopeptide spectrum match and glycoprotein ID and discovery
  • Versatile workflow

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References
  • Chen M. et al. (2017). An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. Nat Commun. May 23;8, 15487.
  • Chen M., et al. (2023). Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation and gives insights into mannose-6-phosphate pathway in cancer. Commun Biol. Jan13;6(1), 48.
  • Mersha F. et al. (2023). Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malay. Sci Rep. May 16;13(1), 7951.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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